Structure of the transcription open complex of distinct σI factors
Jie Li, Haonan Zhang, Dongyu Li, Ya-Jun Liu, Edward A. Bayer, Qiu Cui, Yingang Feng & Ping Zhu
Abstract
Bacterial σIfactors of the σ70-family are widespread in Bacilli and Clostridia and are involved in the heat shock response, iron metabolism, virulence, and carbohydrate sensing. A multiplicity of σIparalogues in some cellulolytic bacteria have been shown to be responsible for the regulation of the cellulosome, a multienzyme complex that mediates efficient cellulose degradation. Here, we report two structures at 3.0 Å and 3.3 Å of two transcription open complexes formed by two σI factors, SigI1 and SigI6, respectively, from the thermophilic, cellulolytic bacterium,Clostridium thermocellum. These structures reveal a unique, hitherto-unknown recognition mode of bacterial transcriptional promoters, both with respect to domain organization and binding to promoter DNA. The key characteristics that determine the specificities of the σI paralogues were further revealed by comparison of the two structures. Consequently, the σIfactors represent a distinct set of the σ70-family σ factors, thus highlighting the diversity of bacterial transcription.
附件下载：
Structure of the transcription open complex of distinct σIfactors,Nat Commun, 13 Oct 2023
Nature Communications, 13 October, 2023, DOI：https://doi.org/10.1038/s41467-023-41796-4
Structure of the transcription open complex of distinct σI factors
Jie Li, Haonan Zhang, Dongyu Li, Ya-Jun Liu, Edward A. Bayer, Qiu Cui, Yingang Feng & Ping Zhu
Abstract
Bacterial σIfactors of the σ70-family are widespread in Bacilli and Clostridia and are involved in the heat shock response, iron metabolism, virulence, and carbohydrate sensing. A multiplicity of σIparalogues in some cellulolytic bacteria have been shown to be responsible for the regulation of the cellulosome, a multienzyme complex that mediates efficient cellulose degradation. Here, we report two structures at 3.0 Å and 3.3 Å of two transcription open complexes formed by two σI factors, SigI1 and SigI6, respectively, from the thermophilic, cellulolytic bacterium,Clostridium thermocellum. These structures reveal a unique, hitherto-unknown recognition mode of bacterial transcriptional promoters, both with respect to domain organization and binding to promoter DNA. The key characteristics that determine the specificities of the σI paralogues were further revealed by comparison of the two structures. Consequently, the σIfactors represent a distinct set of the σ70-family σ factors, thus highlighting the diversity of bacterial transcription.
文章链接：https://www.nature.com/articles/s41467-023-41796-4
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