2007.06 - 2011.09 B.S., China Agricultural University

2011.07 - 2015.12 Research Assistant, Key Laboratory of RNA Biology, Institute of Biophysics, CAS

2015.12 - Associate Professor, Key Laboratory of RNA Biology, Institute of Biophysics, CAS

The clustered regularly interspaced short palindromic repeats (CRISPR), together with CRISPR-associated (Cas) proteins form the microbial adaptive immune system that protects against invading phages and plasmids. The CRISPR-Cas system is a RNA-guided immune system found in nearly half of all bacteria studied, as well as in the majority of archaea. Our research in this area includes the molecular mechanism of spacer acquisition, pre-crRNA processing and RNA-guided DNA/RNA cleavage.

1. 2021 - 2024 National Natural Science Foundation of China, General Program

2. 2018 - 2020 National Natural Science Foundation of China, Young Scientists Program

1. Huang X, Sun W, Cheng Z, Chen M, Li X,Wang J, Sheng G*, Gong W* and Wang Y*. Structural basis for two metal-ion catalysis of DNA cleavage by Cas12i2.Nature Communications, 2020.10, online.

2. Sun W^#, Yang J^#, Cheng Z^#, Amrani N, Liu C, Wang K, Ibraheim R, Edraki A, Huang X, Wang M,Wang J, Liu L, Sheng G, Yang Y, Lou J, Sontheimer E*, and Wang Y*. Structures of Neisseria meningitidis Cas9 Complexes in Catalytically Poised and Anti-CRISPR-Inhibited States.Molecular Cell, 2019, 76(6): 938-952.

3. You L, Ma J,Wang J, Artamonova D, Wang M, Liu L, Xiang H, Severinov K, Zhang X*, Wang Y*. Structure Studies of the CRISPR-Csm Complex Reveal Mechanism of Co-transcriptional Interference.Cell2019, 176, 239–253.

4. Yin M,Wang J, Wang M, Li X, Zhang M, Wu Q*, Wang Y*. Molecular mechanism of directional CTCF recognition of a diverse range of genomic sites.Cell Res2017, 27:1365-1377.

5. Liu L^#, Li X^#, Ma J^#, Li Z^#, You L,Wang J, Wang M, Zhang X*, Wang Y*. The Molecular Architecture for RNA-Guided RNA Cleavage by Cas13a.Cell2017, 170:714-726 e710.

6. Liu L, Li X,Wang J, Wang M, Chen P, Yin M, Li J, Sheng G, Wang Y*. Two Distant Catalytic Sites Are Responsible for C2c2 RNase Activities.Cell2017, 168:121-134 e112.

7. Liu L, Chen P, Wang M, Li X,Wang J, Yin M, Wang Y*. C2c1-sgRNA Complex Structure Reveals RNA-Guided DNA Cleavage Mechanism.Mol Cell2017, 65:310-322.

8. Sheng G, Gogakos T,Wang J, Zhao H, Serganov A, Juranek S, Tuschl T, Patel DJ*, Wang Y*. Structure/cleavage-based insights into helical perturbations at bulge sites within T. thermophilus Argonaute silencing complexes.Nucleic Acids Res2017, 45:9149-9163.

9.Wang J^#, Ma J^#, Cheng Z, Meng X, You L, Wang M, Zhang X*, Wang Y*. A CRISPR evolutionary arms race: structural insights into viral anti-CRISPR/Cas responses.Cell Res2016, 26:1165-1168. （#共同第一作者）

10.Wang J^#, Li J^#, Zhao H, Sheng G, Wang M, Yin M, Wang Y*. Structural and Mechanistic Basis of PAM-Dependent Spacer Acquisition in CRISPR-Cas Systems.Cell2015, 163:840-853. （#共同第一作者）

11. Zhao H, Sheng G,Wang J, Wang M, Bunkoczi G, Gong W, Wei Z, Wang Y*. Crystal structure of the RNA-guided immune surveillance Cascade complex in Escherichia coli.Nature2014, 515:147-150.

12. Sheng G^#, Zhao H^#,Wang J, Rao Y, Tian W, Swarts DC, van der Oost J, Patel DJ*, Wang Y*. Structure-based cleavage mechanism of Thermus thermophilus Argonaute DNA guide strand-mediated DNA target cleavage.Proc Natl Acad Sci U S A2014, 111:652-657.

(From Jiuyu Wang, November 2, 2021)