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Zheng Zhou, Ph.D, Prof.

Principal Investigator
National Laboratory of Biomacromolecules, IBP


Research Interests: Structure and dynamics of nucleosome or nucleosome sub-complexes


Email: zhouzh@ibp.ac.cn


Tel: 010-64889862


Address: 15 Datun Road, Chaoyang District, Beijing, 100101, China


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Biography

1992 - 1999 Hunan Normal University, B.Sc & M.Sc in Biochemistry and Molecular Biology

1999 - 2003 Shanghai Institute of Biochemistry and Cell biology, Chinese Academy of Sciences, Ph.D. in Biochemistry and Molecular Biology

2003 - 2011 National Institutes of Health, National Cancer Institute, Visiting / Research Fellow

2011 - Principal Investigator, National Laboratory of Biomacromolecules. Institute of Biophysics, Chinese Academy of Sciences

Awards
Membership in Academies & Societies
Research Interests

Epigenetic regulation controls dynamic changes in chromatin structure to modulate cellular events related to DNA accessibility, such as transcription, replication, recombination, and repair, etc. Histone variants regulate the dynamic changes of chromatin structure, serving as a key player in epigenetic regulation. Research in my lab focuses on histone variants function and their links to human diseases. We sought to address how histone H2A variants regulate chromatin structure and gene expression in concert with histone chaperone, post-translational modifications, and chromatin remodeller, aiming to understand the fundamental principles of establishment and maintenance of chromatin states that involve histone variants and other epigenetic marks.

We combine multiple structural biology approaches including X-ray crystallography, NMR spectroscopy, and Cryo-Electron Microscopy in our study. We aim to understand the mechanisms underlying the following processes: (1) nucleosome editing (exchange of histone H2A.Z variant), and (2) DNA double-strand break repair pathway choice. The ultimate goal of our study is to understand the role of histone variants and epigenetic regulators in control of chromatin dyanmics and human diseases.

Grants
Selected Publications

1. Huang L, Wang Y, Long H, Zhu H, Wen Z, Zhang L, Zhang W, Guo Z, Wang L, Tang F, Hu J, Bao K, Zhu P, Li G,Zhou Z. (2023) Structural insight into H4K20 methylation on H2A.Z-nucleosome by SUV420H1.Mol Cell. In press.

2. Chen J, Lu Z, Gong W, Xiao X, Feng X, Li W, Shan S, Xu D,Zhou Z. (2022) Epstein-Barr virus protein BKRF4 restricts nucleosome assembly to suppress host antiviral response.Proc Natl Acad Sci USA.119 (37): e2203782119.

3. Shi L, Huang L, Long H, Song A,Zhou Z. (2022) Structural basis of nucleosomal H4K20 methylation by methyltransferase SET8.FASEB J. 36(6):e22338.

4. Dai L, Dai Y, Han J, Huang Y, Wang L, Huang J,Zhou Z.(2021) Structural insight into BRCA1-BARD1 complex recruitment to damaged chromatin.Mol Cell. 81(13): 2765-2777.

5. Dai L, Xiao X, Pan L, Shi L, Xu N, Zhang Z, Feng X, Ma L, Dou S, Wang P, Zhu B, Li W,Zhou Z.(2021) Recognition of the inherently unstable H2A nucleosome by Swc2 is a major determinant for unidirectional H2A.Z exchange.Cell Rep. 35(8):109183.

6. Zhou N, Shi L, Shan S,Zhou Z.(2021) Molecular basis for the selective recognition and ubiquitination of centromeric histone H3 by yeast E3 ligase Psh1.J Genetics Genomics. 48(6):463-472.

7. Zhou M, Dai L, Li C, Shi L, Huang Y, Guo Z, Wu F, Zhu P,Zhou Z.(2021) Structural basis of nucleosome dynamics modulation by histone variants H2A.B and H2A.Z.2.2,EMBO J. 40(1): e105907.

8. Huang Y, Sun L, Pierrakeas L, Dai L, Pan L, Luk E,Zhou Z.(2020) Role of a DEF/Y motif in histone H2A-H2B recognition and nucleosome editing.Proc Natl Acad Sci USA. 117(7): 3543–3550.

9. Dai Y, Zhang F, Wang L, Shan S, Gong Z,Zhou Z.(2019) Structural basis for shieldin complex subunit 3-mediated recruitment of the checkpoint protein REV7 during DNA double-strand break repair.J Biol Chem.295(1):250-262.

10. Dai L, Xu N,Zhou Z.(2019) NMR investigations on H2A-H2B heterodimer dynamics conferred by histone variant H2A.Z.Biochem Biophys Res Commun. 518(4):752-758.

11. Wang Y, Liu S, Sun L, Xu N, Shan S, Wu F, Liang X, Huang Y, Luk E, Wu, C,Zhou Z. (2019) Structural insights into histone chaperone Chz1-mediated H2A.Z recognition and histone replacement.PLoS Biol.17(5): e3000277.

12. Dai Y, Zhang A, Shan S, Gong Z,Zhou Z.(2018) Structural basis for recognition of53BP1 tandem Tudor domain by TIRR.Nat Commun. 9(1):2123.

13. Dai L, Xie X,Zhou Z. (2018) Crystal structure of the histone heterodimer containing histone variant H2A.Bbd.Biochem Biophys Res Commun. 503(3):1786-1791.

14. Liang X, Shan S, Pan L, Zhao J,Ranjan A, Wang F, Zhang Z, Huang Y, Feng H, Wei D, Huang L, Liu X, Zhong Q, Lou J, Li G, Wu C,Zhou Z. (2016) Structural basis of H2A.Z recognition by SRCAP chromatin-remodeling subunit YL1.Nat Struct Mol Biol. 23(4):317-325.

15. Mao Z, Pan L, Wang W, Sun J, Shan S, Dong Q, Liang X, Dai L, Ding X, Chen S, Zhang Z, Zhu B,Zhou Z.(2014) Anp32e, a higher eukaryotic histone chaperone directs preferential recognition for H2A.Z.Cell Res.24(4):389–399.

Invited reviews

1. Xiao S, Wang Y, Shan S,Zhou Z. (2023) The interplay between viral molecular mimicry and host chromatin dynamics.Nucleus. 14(1): 2216560.

2. Huang Y, Dai Y,Zhou Z. (2020) Mechanistic and structural insights into histone H2A-H2B chaperone in chromatin regulation.Biochem J. 477 (17): 3367-3386. (review)

3. Huang Y,Zhou Z. (2018) Recent progress in histone chaperones associated with H2A-H2B type histones.Prog Biochem Biophys. 45(9): 971-980. (review)

4. Zhou J, Feng X,Zhou Z. (2015) Chromatin assembly of histone variants.Prog Biochem Biophys.42(11):1003~1008. (review)

(From Zheng Zhou, July 5, 2023)

Contact Us

Tel: 010-64889872

E-Mail: webadmin@ibp.ac.cn

Address: No 15 Datun Road, Chaoyang District, Beijing

Postcode: 100101

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